Notch1, Cytoplasmic Domain (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

The Notch signaling pathway, first identified in Drosophila, regulates the generation of differentiated cells from a population of undifferentiated progenitors. The Notch1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta-like-3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kD) which localizes to the nucleus to interact with DNA binding proteins (RB-Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes.\n\nApplications:\nSuitable for use in Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot Analysis: 2-4ug/ml Detected Notch1 in a TENT buffer lysate from mouse L fibroblasts transfected with rat Notch1. 20ug of lysate from mouse L fibroblasts transfected with Notch1 was resolved by electrophoresis, transferred to nitrocellulose and probed with N5375-05 Notch1 (4ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5375-05

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Blaumueller, C.M., et al., Cell 90: 281

Additional Info

Recognizes rat Notch1 at 300kD, the cytoplasmic fragment of 120kD and an additional band at 200kD. Species Crossreactivity: Rat. Species sequence homology: mouse.