Notch1 (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1)

The Notch signaling pathway, first identified in Drosophila, regulates the generation of differentiated cells from a population of undifferentiated progenitors. The Notch 1 protein is a single pass transmembrane receptor activated through the Delta (Dl) and Serrate (Ser) ligands in Drosophila (and their mammalian counterparts Delta-like-3 and Jagged 1 and 2). Activation results in the proteolytic cleavage of the cytoplasmic portion of Notch (120kD) which localizes to the nucleus to interact with DNA binding proteins (RB-Jk/KBF2/CBF1). The interaction of Notch with these proteins converts them from transcriptional repressors to activators, thus affecting the expression of a variety of target genes.\nDuring maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain. Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744. The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription.\n\nSource: Syrian hamster\n\nApplications: \nSuitable for use in Flow cytometry, ELISA, Western Blot, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000 detects Notch 1 in RIPA lysates from 40ug of mouse C2C12 muscle myoblast cells. C2C12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N5375-06 (1:250). Proteins were visualized using a goat anti-hamster secondary antibody conjugated to HRP (eg. I1903-04T) and a chemiluminescence detection system. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5375-06

Size

200ul

Applications

ELISA, FC, ICC, WB

Reactivities

Mouse, Rat

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Robey, E., Curr. Opin. Mol. Cell Bio. 7: 551

Additional Info

Recognizes mouse Notch 1 at Mr 300kD. Additional proteins were detected at Mr 230kD and Mr 160kD, believed to be the processed extracellular fragments of Notch 1. Species Crossreactivity: rat