Notch2 (Notch homolog 2, Drosophila, hN2)

Notch signaling plays a key role in the normal development of many tissues and cell types, through diverse effects on differentiation, survival, and/or proliferation that are highly dependent on signal strength and cellular context. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Notch family members share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Multiple human notch proteins (NOTCH1, NOTCH2, NOTCH3 and NOTCH4) have been identified and they function as a receptors for membrane bound ligands. Notch signaling is also linked to tumorigenesis as first demonstrated by the identification of a recurrent t(7;9)(q34;q34.3) chromosomal translocation involving the human Notch1 gene that is found in a small subset of human pre-T-cell acute lymphoblastic leukemias (T-ALL). Since this discovery, aberrant Notch signaling has been suggested to be involved in a wide variety of human neoplasms.\n\nApplications:\nSuitable for use in ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nAntibody may be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-15

Size

50ug

Applications

ELISA

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Schnabel M, Fichtel I, Gotzen L, Schlegel J: Differential expression of Notch genes in human osteoblastic cells. Int. J. Mol. Med. 9(3): 229-232 (2002).\n2. Kojika S, Griffin JD: Notch receptors and hematopoiesis. Exp. Hematol. 29(9): 1041-1052 (2001). Review.\n3. Larsson C, Lardelli M, White I, Lendahl U: The human NOTCH1, 2, and 3 genes are located at chromosome positions 9q34, 1p13-p11, and 19p13.2-p13.1 in regions of neoplasia-associated translocation. Genomics 24(2): 253-258 (1994).\n4. Blaumueller CM, Qi H, Zagouras P, Artavanis-Tsakonas S: Intracellular cleavage of Notch leads to a heterodimeric receptor on the plasma membrane.Cell 90(2): 281-291 (1997).

Additional Info

Recognizes human Notch 2.