Notch2 (Notch Homolog 2, Drosophila, hN2)

Supplier:

United States Biological

Cat no: N5375-17

Notch2 (Notch Homolog 2, Drosophila, hN2)

Human Notch-2 is a 300kD member of the notch family of transmembrane (TM) proteins. It is synthesized as a 2446 amino acid (aa) type I TM glycoprotein that undergoes Golgi processing to generate a 180kD disulfide-linked extracellular domain (ECD) (likely aa25-1538) and a 110kD membrane bound truncated segment (likely aa1539-2471). Upon binding to Delta1, Jagged1, or Jagged2, the 110kD segment undergoes two cleavages, the second which generates an NICD (notch intracellular domain) (aa1697-2471), a potential nuclear transcription factor. In the nucleus, NICD regulates CSL pathways. When NICD is phosphorylated, it is deactivated. \n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml; With an appropriate colorimetric detection system, the detection limit for rhNotch-2 ICD is ~5ng/lane under non-reducing and reducing conditions.\nELISA: 0.5-1ug/ml; Detection limit for rhNotch-2 ICD is ~12ng/well.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5375-17

Size

100ug

Applications

ELISA, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 0.2ml sterile 40-50% glycerol, PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human Notch-2 intracellular domain (ICD). Shows no crossreactivity with rhNotch-1 ICD, rrNotch-1 ECD, rrNotch-2 ECD, rhNotch-3 ECD, or rhNotch-4 ICD. Species Sequence Homology: Canine - 94%; mouse - 89%.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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