NOVA 1 RNA Binding Protein (Euro-oncological ventral antigen 1, Neuro-oncological ventral antigen 1, Nova-1, Onconeural ventral antigen-1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1)

The NOVA1 gene encodes a neuron-specific RNA-binding protein that is inhibited by paraneoplastic antibodies (Buckanovich et al., 1996). See also NOVA2 (601991).\nTo identify Nova RNA targets, Ule et al. (2003) developed a method to purify protein-RNA complexes from mouse brain using ultraviolet crosslinking and immunoprecipitation (CLIP). Thirty-four transcripts were identified multiple times by Nova CLIP. Three-quarters of these encode proteins that function at the neuronal synapse, and one-third are involved in neuronal inhibition. Splicing targets confirmed in Nova-null mice include c-Jun N-terminal kinase-2 (602896), neogenin (601907), and gephyrin (603930); the last encodes a protein that clusters inhibitory gamma-aminobutyric acid and glycine receptors, 2 previously identified Nova splicing targets. Thus, CLIP revealed that Nova coordinately regulates a biologically coherent set of RNAs encoding multiple components of the inhibitory synapse, an observation that may relate to the cause of abnormal motor inhibition in paraneoplastic opsoclonus myoclonus ataxia (POMA).\nBy analyzing alternative splicing in brains of Nova1 -/- and Nova2 -/- mice, Ule et al. (2005) identified Nova-dependent alternatively spliced transcripts. Of the 40 Nova-spliced transcripts with defined brain function, 34 encoded proteins that act at the synapse (neurotransmitter receptors, cation channels, adhesion and scaffold proteins), and 8 encoded proteins involved in axon guidance. Of the 35 proteins with known interaction partners, 26 (74%) interact with each other.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution\nELISA (serum titer): 1:62,500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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