Nova 1 RNA Binding Protein (Euro-oncological ventral antigen 1, Neuro-oncological ventral antigen 1, Nova-1, Onconeural ventral antigen-1, Paraneoplastic Ri antigen, Ventral neuron-specific protein 1)

Gene map locus 14q\n\nUsing antisera from patients with a paraneoplastic neurologic disorder involving the subcortical motor system, Buckanovich et al. (1993) identified a gene that they termed NOVA1. The NOVA1 gene encodes a neuron-specific RNA-binding protein that is inhibited by paraneoplastic antibodies (Buckanovich et al., 1996). See also NOVA2 (601991).\n\nTo identify Nova RNA targets, Ule et al. (2003) developed a method to purify protein-RNA complexes from mouse brain using ultraviolet crosslinking and immunoprecipitation (CLIP). Thirty-four transcripts were identified multiple times by Nova CLIP. Three-quarters of these encode proteins that function at the neuronal synapse, and one-third are involved in neuronal inhibition. Splicing targets confirmed in Nova-null mice include c-Jun N-terminal kinase-2 (602896), neogenin (601907), and gephyrin (603930); the last encodes a protein that clusters inhibitory gamma-aminobutyric acid and glycine receptors, 2 previously identified Nova splicing targets. Thus, CLIP revealed that Nova coordinately regulates a biologically coherent set of RNAs encoding multiple components of the inhibitory synapse, an observation that may relate to the cause of abnormal motor inhibition in paraneoplastic opsoclonus myoclonus ataxia (POMA).\n\nBy analyzing alternative splicing in brains of Nova1 -/- and Nova2 -/- mice, Ule et al. (2005) identified Nova-dependent alternatively spliced transcripts. Of the 40 Nova-spliced transcripts with defined brain function, 34 encoded proteins that act at the synapse (neurotransmitter receptors, cation channels, adhesion and scaffold proteins), and 8 encoded proteins involved in axon guidance. Of the 35 proteins with known interaction partners, 26 (74%) interact with each other.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: A 1:1000-1:5000 of N5376-01 detected Nova-1 in RIPA lysates of mouse spinal cord. Nova-1 was also detected in mouse forebrain RIPA lysates and mouse total brain extracts. Spinal cord lysate (20ug) and wild type were prepared and resolved by electrophoresis, transferred to nitrocellulose and probed wth N5376-01. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5376-01

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M sodium chloride and 0.05% sodium azide before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Jensen, K.B., et al., Neuron 25: 359-371, 2000.\n2. Ule, J., et al., Science 302: 1212-1215, 2003.\n3. Yang, Y.Y., et al., Proc. Natl. Acad. Sci. USA 95: 13254-13259, 1998.

Additional Info

Recognizes mouse Nova-1, Mr 53kD. The lower MW isoform of Nova-1 was also slightly detected, Mr 50kD. Species Crossreactivity: rat. Species sequence homology: Human and chicken.