Novel Organic Cation Transporter 1 (OctN1)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N- and C-terminus.\nA novel transporter termed OctN1, has been cloned and characterized from human fetal liver, which carries a nucleotide birding site motif. OctN1 (human 551aa, mouse 553aa) shares ~32% homology with Oct1-3. It is strongly expressed in adult kidney, trachea, bone marrow, and fetal liver, and several tumor cells, but not in adult human liver. OctN1 mediated uptake of TEA in pH dependent manner.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. Coat ELISA plates with control peptide at 1ug/ml.\nWestern Blot: 1-10ug/ml (ECL).\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN5376-50: Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N5376-51

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 100mM Tris-HCl, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kekuda, R., et al., J. Biol. Chem. 273: 15,971-15,979 (1998). 2. Verhaagh, S., et al., Genomics 55: 209-218 (1999). 3. Gruendemann, D., et al., Nat. Neurosci. 1: 349-351 (1998). 4. Wu, X., et al., J. Biol. Chem. 273: 32,776-32,786 (1998). 5. Koespell, H., Ann. Rev. Physiol. 60: 243-266 (1998, review).

Additional Info

Recognizes mouse Novel Organic Cation Transporter 1. No significant sequence homology is detected with other Octs or OctNs. Species Sequence Homology: Rat: 88%; Human: 61%.