Novel Organic Cation Transporter 2 (OctN2)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues. OCT superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus. OCTN2, a structural homolog of OCTN1, has been identified as carnitine transporter. OCTN2 (human/rat/mouse 557 aa) shares ~76% homology with OCTN1. It is strongly expressed in human kidney, skeletal muscle, heart, and placental in adult humans. In rat, OCTN2 expression was found in the proximal and distal tubules and in the glomeruli in the kidney, in the myocardium, valves, and arterioles in the heart, in the labyrinthine layer of the placenta, and brain (cortex, hippocampus, and cerebellum). OCTN2 mediates uptake of carnitine in a Na+-dependent manner, whereas other organic cations are transported without Na+.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 using ECL.\nELISA: 1:10,000-1:50,000. Use 1ug/ml of N5376-54B Control Peptide to coat ELISA plates.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5376-54

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Wu X et al (1998) BBRC 246: 589-595; 2. Tamai I et al (1998) J. Biol. Chem. 273: 20378-20382; 3. Tang NLS et al (1999) Hum. Mol. Genet. 8, 655-660; 4. Wu X et al (1999) J. Pharamcol Exp. Therap. 290: 1482-1492; 5. Koespell H (1998) Ann Rev. Physiol. 60: 243-266 (review).

Additional Info

Recognizes mouse OCTN2. Species Sequence Homology: rat, 88%; human, 76%. No significant sequence homology is detected with other OCTs or OCTNs.