Novel Organic Cation Transporter 2 (OCTN2)

Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (OCTs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (OCT1-3) and H+-dependent transporters (OCTN1-3) have been cloned and characterized from various tissues. OCT superfamily of proteins shares high degree of sequence homology and display up to 12 transmembrane domains with cytoplasmic N and C-terminus.\nOCTN2, a structural homolog of OCTN1, has been identified as carnitine transporter. OCTN2 (human/rat/mouse 557 aa) shares ~76% homology with OCTN1. It is strongly expressed in human kidney, skeletal muscle, heart, and placental in adult humans. In rat, OCTN2 expression was found in the proximal and distal tubules and in the glomeruli in the kidney, in the myocardium, valves, and arterioles in the heart, in the labyrinthine layer of the placenta, and brain (cortex, hippocampus, and cerebellum). OCTN2 mediated uptake of carnitine in a Na+-dependent manner, whereas other organic cations were transported without Na+.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL).\nELISA: 0.5-1.0ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide:\nN5376-54B: Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1). \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

N5376-54A

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

(1). Wu X et al (1998) BBRC 246, 589-595; Tamai I et al (1998) J. Biol. Chem. 273, 20378-20382; tang NLS et al (1999) Hum. Mol. Genet. 8, 655-660; Wu X et al (1999) J. Pharamcol Exp. Therap. 290, 1482-1492; Koespell H (1998) Ann Rev. Physiol. 60, 243-266 (review).

Additional Info

Recognizes mouse OCTN2. No significant sequence homology is detected with OCTs or OCTNs.