NP-5 (Alpha Defensin 5, Defensin 5, DEF5, Defensin alpha 5, Defensin alpha 5 Paneth Cell-specific, Defensin alpha 5 Preproprotein, DEFA5, HD5, HD-5, HNP5, HNP-5, MGC129728)

Human neutrophil alpha-defensins (also designated HNPs) are small, cationic, cysteine-rich antimicrobial proteins that play important roles in innate immunity against infectious microbes such as bacteria, fungi and enveloped viruses. Alpha-defensins are synthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7. In the mature form, NP-5 is cleaved at residue 63 (alanine). Paneth cells in small intestinal crypts secrete the alpha-defensins, which are also termed cryptidins in mice. Alpha-defensins 5 and 6 probably contribute to innate defense of the GI mucosal surface by protecting against microbial invasion in states of intestinal inflammation.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot. Dot Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (FFPE): 5-10ug/ml\nImmunohistochemistry (Frozen): 5-10ug/ml\nWestern Blot: 1:1000\nImmunofluorescence (IC): 1:200-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5378-07B

Size

100ul

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography

Isotype

IgG2b

References

1. Porter, EM, et al., Infection and Immunity 65: 2389-2395 (1997). 2.Wehkamp, J. et al. PNAS. 102(50): 18129-34 (2005) [Western blot, Immunohistochemistry]\n3. Wehkamp, J. et al. FEBS Letters: 580 (2006) [Immunohistochemistry, Immunofluorescence]

Additional Info

Recognizes human HD5 at ~10kD. No crossreactivity is observed with HD6, lysozyme or sPLA2.