NP-5 (alpha-Defensin 5, HD5)

HD5 is an antimicrobial peptide produced by Paneth cells, specialized epithelial cells of the small intestinal crypt. HD5 is stored in the Paneth cells as propeptide (aa 20-94) and released as mature peptide after trypsin digestion, which is co-secreted from the Paneth cell granules. The mature HD5 (aa 63-94) is protected from further digestion by the proteolysis enzyme. HD5 functions in maintaining the correct microbial balance in the intestine. Transgenic mice that express HD5 are protected from lethal challenge caused by Salmonella typhimurium. Expression levels of HD5 are negatively correlated to intestinal infection. HD5 is a useful marker for intestinal metaplasia of the upper gastrointestinal tract. HD5 expression has also been detected in the upper half of the stratified squamous epithelium of the vagina and ectocervix with increasing intensity toward the lumen. Secreted HD5 is detectable in cervicovaginal lavages, with highest concentrations during the secretory phase of the menstrual cycle. \n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, and ELISA. Other appllications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (frozen): 1:50\nWestern Blot: 1:50 \nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5381

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

Kelly, P et al; Reduced gene expression of intestinal alpha-defensins predicts diarrhea in a cohort of \nAfrican adults. J Inf Dis 2006, 193: 1464 Shen, B et al; Human defensin 5 expression in intestinal metaplasia of the upper gastrointestinal tract. J Clin Pathol 2005, 58: 687 Kelly, P et al; Paneth cell granule depletion in the human small intestine under infective and nutritional stress. Clin Exp Immunol 2004, 135: 303.

Additional Info

Recognizes human Alpha-Defensin 5. No cross reactivity with HD6, lysozyme, or sPLA2 could be detected.