NP-5 (alpha Defensin-5, HNP-5)

Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35 aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely b-sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms. In leukocytes, these peptides are stored in cytoplasmic granules and are released into phagolysosomes where they contribute to the killing of engulfed microorganisms. The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated alpha and beta based on distinctive, although similar, tri-disulfide linkages in the peptides. b-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six a-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two b-defensins, HBD-1 and HBD-2, have been identified.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 using ECL technique\nELISA: 1:10,000-50,000. Coat ELISA plates with 1ug/ml control peptide.\nOptimal dilution to be determined by the researcher.\n\nControl Peptide: N5378-07\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N5378-07G

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid (serum) containing 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

ones DE et al (1992) J. Biol. Chem. 267, 23216-23225; Wilson CL et al (1999) Science 286, 113-117;Mallow EB et al (1996) J. Biol. Chem. 271, 4038-4045; Ganz T and Lehrer RI (1998) Current Opinion Biol. 10, 41-44;. \n

Additional Info

Recognizes human alpha defensin-5. No significant homology with other alpha-defensins.