NPAS3, ID (Neuronal PAS Domain-containing Protein 3, Neuronal PAS3, Basic-helix-loop-helix-PAS Protein MOP6, Class E Basic Helix-loop-helix Protein 12, bHLHe12, Member of PAS Protein 6, PAS Domain-containing Protein 6, BHLHE12, MOP6, PASD6)

Neuronal PAS domain protein 3 (NPAS3) is a brain- enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3- null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M4591-33D

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Brunskill EW, et al (1999) Mech. Dev. 88:237-41. 2. Kamnasaran D, et al J. Med. Genet. 40:325-32. 3. Brunskill EW, et al (2005) Euro. J. Neurosci. 22:1265-76.

Additional Info

Recognizes human NPAS3. Species Crossreactivity: mouse and rat.