NPAS3 (Neuronal PAS Domain Protein 3, Neuronal PAS3)

Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium (1) and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia (2). It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities (3). Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2 ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M4591-34

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Brunskill EW, Witte DP, Shreiner AB et al. . Mech. Dev. 1999; 88:237-41. 2. Kamnasaran D, Muir WJ, Ferguson-Smith MA, et al. . J. Med. Genet. 40:325-32. 3. Brunskill EW, Ehrman LA, Williams MT, et al. . Euro. J. Neurosci. 2005; 22:1265-76.

Additional Info

Recognizes human NPAS3. Species Crossreactivity: Mouse and rat.