NPM (Nucleolar protein NO38, Numatrin, Nucleolar phosphoprotein B23, Nucleophosmin, NPM,Npm1) (Azide free) (HRP)

RNA-associated nucleolar phosphoprotein; involved in ribosome assembly [RGD]\n\nApplications:\nSuitable for use in Western Blot, ELISA.\n\nRecommended Dilution:\nELISA: 1:1000\nWestern Blot: 1:4~60\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C with glycerol. Do not freeze horseradish peroxidase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

031163-HRP

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added (Azide free). Conjugated to HRP.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Protein B23/nucleophosmin/numatrin nuclear dynamics in relation to protein kinase CK2 and apoptotic activity in prostate cells. Wang G, et al. Biochemistry, 2010 May 11. PMID 20387789.\nNucleophosmin may act as an alarmin: implications for severe sepsis. Nawa Y, et al. J Leukoc Biol, 2009 Sep. PMID 19581374.\nDisruption of ATP binding destabilizes NPM/B23 and inhibits anti-apoptotic function. Choi JW, et al. BMB Rep, 2008 Dec 31. PMID 19123973.\nNuclear Akt interacts with B23/NPM and protects it from proteolytic cleavage, enhancing cell survival. Lee SB, et al. Proc Natl Acad Sci U S A, 2008 Oct 28. PMID 18931307.\nEbp1 association with nucleophosmin/B23 is essential for regulating cell proliferation and suppressing apoptosis. Okada M, et al. J Biol Chem, 2007 Dec 14. PMID 17951246.

Additional Info

Human

Alternative Names

Nucleolar protein NO38, Numatrin, Nucleolar phosphoprotein B23, Nucleophosmin, NPM,Npm1