NPTXR (Neuronal Pentraxin Receptor, NPR)

Supplier:

United States Biological

Cat no: N5379-08R

NPTXR (Neuronal Pentraxin Receptor, NPR)

NPTXR (Neuronal Pentraxin Receptor; also NPR) is a 65kD, type II transmembrane glycoprotein that binds Neuronal Pentraxins 1 and 2. It is expressed on cerebellar Purkinje and granule cells, and hippocampal neurons of the CA1, CA3 and dentate gyrus regions. Human NPTXR is 499 amino acids (aa) in length. It contains a two aa cytoplasmic tail, a 21aa transmembrane segment, and a 476aa extracellular region with one Pentraxin domain (aa295-499). One alternate start site exists at Met12, and a 55kD soluble form (possibly beginning at Gln56) may be generated by proteolytic cleavage. \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1ug/ml; Detection limit for rhNPTXR is ~0.3ng/well.\nWestern Blot: 0.1-0.2ug/ml; Detection limit for rhNPTXR is ~20ng/lane and 1ng/lane under non-reducing and reducing conditions, respectively.\nImmunohistochemistry: 3-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N5379-08R

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human NPTXR, extracellular domain. Displays less than 5% crossreacitvity with rhNPTX1 and rhNPTX2. Species Sequence Homology: Mouse - 87%.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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