NQO1 (NAD(P)H Dehydrogenase Quinone 1, Azoreductase, Cytochrome b-5 Reductase, DHQU, Diaphorase (NADH/NADPH) (Cytochrome b-5 Reductase), Diaphorase 4, DIA4, Dioxin Inducible 1, DT Diaphorase, DTD, Menadione Reductase, NAD(P)H Menadione Oxidoreductase 1 Di

NQO1 (NAD(P)H:quinone oxidoreductase 1) is a 31kD cytosolic flavoenzyme and member of the NAD(P)H dehydrogenase (quinone) family, involved in detoxification, which catalyzes the two-electron reduction of quinones to hydroquinones. NQO1 is expressed in a wide range of tissues and is overexpressed in many human tumors including lung, brain, liver, colon and breast. The ability of NQO1 to bioactivate anti-tumor quinones including mitomycin C and diaziquone, has become a focus area for chemotherapeutic studies. Mutations in the NQO1 gene are associated with an increased risk of certain cancers, and an increased risk of leukaemia has been associated with diminished NQO1 activity and the NQO1 *2 allele.\n\nApplications:\nSuitable for use in Immunoprecipitation, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100-1:1,000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHuman lung\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5379-50G

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Siegel, D. et al. (1998) Immunohistochemical detection of NAD(P)H:quinone oxidoreductase in human lung and lung tumors. Clin. Cancer Res. 4: 2065-2070. 2. Anwar, A. et al. (2002) Interaction of the molecular chaperone Hsp70 with human NAD(P)H:quinone oxidoreductase 1. J. Biol. Chem. 277: 14060-14067.

Additional Info

Recognizes endogenous levels of total human NQO1. Species Crossreactivity: rat (weakly). Does not react with mouse. Does not cross-react with NQO2.