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NQO2, Recombinant, Human (NAD(P)H Dehydrogenase Quinone 2, DIA6, DHQV, Ribosyldihydronicotinamide Dehydrogenase [Quinone], NRH Dehydrogenase [Quinone] 2, NRH:Quinone Oxidoreductase 2, NMOR2, Quinone Reductase 2, QR2)

Cat no: N5379-60H

NQO2, Recombinant, Human (NAD(P)H Dehydrogenase Quinone 2, DIA6, DHQV, Ribosyldihydronicotinamide Dehydrogenase [Quinone], NRH Dehydrogenase [Quinone] 2, NRH:Quinone Oxidoreductase 2, NMOR2, Quinone Reductase 2, QR2)

NQO2 is a member of the NAD(P)H dehydrogenase (quinone). The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. It is flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. Recombinant human NQO2 protein was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa1-231 of human NQO2 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MAGKKVLIVY AHQEPKSFNG SLKNVAVDEL SRQGCTVTVS DLYAMNFEPR ATDKDITGTL SNPEVFNYGV ETHEAYKQRS LASDITDEQK KVREADLVIF QFPLYWFSVP AILKGWMDRV LCQGFAFDIP GFYDSGLLQG KLALLSVTTG GTAEMYTKTG VNGDSRYFLW PLQHGTLHFC GFKVLAPQIS FAPEIASEEE RKGMVAAWSQ RLQTIWKEEP IPCTAHWHFG Q\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5379-60H

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 10% glycerol.

Purity

~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Wu K., et al. (1997) Arch Biochem Biophys. 347(2):221-8. 2. Jaiswal AK., et al. (1994) J Biol Chem. 269(20):14502-8.

Alternative Names

EC=1.10.99.2

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