NROB1 contains a DNA-binding domain and acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in the gene for NROB1 result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.
Applications:
Suitable for use in ELISA and Western Blot Other applications not tested.
Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml, observed in human ovary and testis lysates on ~50-55kD bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.