NR2B

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease, epilepsy and ischemic neuronal cell death.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml (~180kD). Detects NR2B in rat brain microsomal protein preparation. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nControl:\nMouse Brain Tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

N5380-30

Size

250ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Bliss, T.V.P., Collingridge, G.L., Nature 361: 31-39 (1993). 2. Choi, D.W., Rothman, S.M., Ann. Rev. Neurosci. 13: 171-182 (1990). 3. Constantine-Patton, M., et al., Ann. Rev. Nerosci. 13: 129-154 (1990). 4. Mandich, P., et al., Genomics 22: 216-218 (1994). 5. Sheng, M., et al., Nature 368: 144-147 (1994). 6. Rao, A., et al., J. Neurosci. 18: 1217-1229 (1998). 7. Rao, A., Craig, A.M., Neuron 19: 801-812 (1997). 8. Ehlers, M.D., et al., Cell 84: 745-755 (1996).

Additional Info

Recognizes mouse NR2B at ~180kD. Species sequence homology: Sequence is identical to and may react with aa1437-1456 of mature rat NR2B and aa1465-1484 of human NR3 (containing a N-terminal lysine).