NRG1 (GGF, HGL, HRGA, NDF, SMDF, Pro-neuregulin-1, Membrane-bound Isoform, Neuregulin-1, Acetylcholine Receptor-inducing Activity, Breast Cancer Cell Differentiation Factor P45, Glial Growth Factor, Heregulin, Neu Differentiation Factor, Sensory and Motor

Neuregulin 1 (NRG1) was originally identified as a 44kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization. Other applications not tested.\n\nRecommended Dilution:\nELISA: To detect Human Heregulin-beta1 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.\nWestern Blot: To detect Human Heregulin-beta1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human Heregulin-beta1 is 1.5-3ng/lane, under either reducing or non-reducing conditions.\nImmunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal breast. The recommended concentration is 0.125mg/mL-0.0625mg/mL with an overnight incubation at 4 degrees C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.\nNeutralization: To yield one-half maximal inhibition of the biological activity of Human Heregulin-beta1 (1ng/ml), a concentration of 0.7-1.1ug/ml of this antibody is required.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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