NRP2 (Vascular endothelial cell growth factor 165 receptor 2)

NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of \nreceptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in \nChinese hamster ovary cells. The NRP2 gene is mapped on 2q33.3. The NRP2 gene contains 17 \nexons and spans about 112 kb. Sema3b can act to repulse axons expressing neuropilin-2 and can \nantagonize the repulsive action of Sema3a on axonal growth cones expressing neuropilin-1. This \nprotein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with \nnull mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 \n(PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine\nnumber and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and \nexcitatory synapses in dissociated neurons in vitro, and in Npn2-null brain slices cortical layer V and \ndentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These \ndisparate effects of secreted semaphorins are reflected in the restricted dendritic localization of Npn2 to \napical dendrites and of Npn1 to all dendrites of cortical pyramidal neurons.\n\nUniProt Number:\nO60462\n\nGene ID:\nNRP2\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144995

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Chen, H., Bagri, A., Zupicich, J. A., Zou, Y., Stoeckli, E., Pleasure, S. J., Lowenstein, D. H., Skarnes, \nW. C., Chedotal, A., Tessier-Lavigne, M.Neuropilin-2 regulates the development of select cranial \nand sensory nerves and hippocampal mossy fiber projections. Neuron 25: 43-56, 2000.\n2. Gong, S., Zheng, C., Doughty, M. L., Losos, K., Didkovsky, N., Schambra, U. B., Nowak, N. J., \nJoyner, A., Leblanc, G., Hatten, M. E., Heintz, N.A gene expression atlas of the central nervous \nsystem based on bacterial artificial chromosomes. Nature 425: 917-925, 2003.\n3. Rossignol, M., Beggs, A. H., Pierce, E. A., Klagsbrun, M. Human neuropilin-1 and neuropilin-2 map \nto 10p12 and 2q34, respectively.Genomics 57: 459-460, 1999.

Additional Info

Recognizes human, mouse and rat NRP2. No crossreactivity with other proteins.