NT-3, Recombinant, Human (Neurotrophin-3, Neurotrophic factor, Nerve growth factor-2, NGF-2, HGNF)

NT-3 is a neurotrophic factor structurally related to beta-NGF, BDNF, and NT-4.These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors.NT-3 promotes the growth and survival of nerve and glial cells.The amino acid sequences of human, murine and rat NT-3 are identical.Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (240 total amino acid residues).Human and Mouse NT-3 sequences are identical.\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50ng/ml.\n\nAA Sequence:\nMYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT\n\nCrossreactivity:\nChicken, Bovine, Canine, Frog, Hamster, Mouse, Pig, Rat\n\nQuality Control:\nVerified by N-terminal and Mass Spectrometry analyses (when applicable).\n\nEndotoxin:\n<0.1ng/ug of protein (<1EU/ug).\n\nProtein Content:\nVerified by UV Spectroscopy and/or SDS-PAGE gel.\n\nStorage and Stability:\nStore lyophilized products at -20 degrees C. For reconstituted solutions of most products, we recommend short-term storage at 4 degrees C. For longer term storage the protein solution should be stored with a carrier protein (eg. 0.1% BSA) in working aliquots and stored frozen at -20 degrees C. Additional freeze/thaw cycles may cause some denaturation of the protein.

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