Nuclear Factor I/X (MLZE) ELISA Kit

Cat no: ABIN435482

Supplier: Antibodies-online

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Nuclear Factor I/X (MLZE) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor I/X (NFIX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor I/X (NFIX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor I/X (NFIX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN435482
Size:	96 tests
Gene:	81524
Antigen:	NFIX
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	0.156-10 ng/mL
Sample type:	Tissue Homogenate,Biological Fluids
Entrez gene:	81524

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Nuclear Factor I/X (MLZE) ELISA Kit

Cat no: ABIN435482

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