Nuclear Factor-KB P65 (NFkBP65) ELISA Kit

Cat no: ABIN414493

Supplier: Antibodies-online

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Nuclear Factor-KB P65 (NFkBP65) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN414493
Size:	96 tests
Antigen:	Transcription Factor P65 (REL-A)
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D. Value of 20 replicates of the zero standard added by their three standard deviations.
Assay range:	0.156-10 ng/mL
Sample type:	Tissue Homogenate,Cell Lysate,Biological Fluids

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Nuclear Factor-KB P65 (NFkBP65) ELISA Kit

Cat no: ABIN414493

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