Nucleophosmin (NPM, B23, MGC104254, NO38, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Nucleophosmin/B23.2, Nucleoplasmin Family Member 1, NPM1, Numatrin, TRK Fused Gene)

B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E. This phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor alpha (RAR alpha). As a consequence, a fusion protein (60-70kD) is produced containing parts of the two genes. Monoclonal antibody reacting specifically with B23 is a useful tool for the study of the involvement of B23 in nuclear functions, cytoplasmic/nuclear trafficking, and centrosome duplication.\n\nCellular Localization: Nuclear\n\nPositive Control: NIH3T3 whole cell lysate.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2-4ug/m\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N7100-20C

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Dnk

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 15mM sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG1

References

Chan PK et al. Amino acid sequence of a specific antigenic peptide of protein B23. J Biol Chem 261:14335-41 (1986). <PUBMED:2429957>Yung BY et al. Translocation of nucleolar phosphoprotein B23 (37 kDa/pI 5.1) induced by selective inhibitors of ribosome synthesis. Biochim Biophys Acta 826:167-73 (1985). <PUBMED:3907710>Ochs R et al. Localization of nucleolar phosphoproteins B23 and C23 during mitosis. Exp Cell Res 146:139-49 (1983). <PUBMED:6345184>

Additional Info

Recognizes both phosphorylated and unphosphorylated B23 molecule at ~37kD. Species Crossreactivity: Human, Mouse, Rat, bovine, canine, Donkey and Hamster.