Occludin (FITC)

Occludin is localized at tight junctions of both epithelial and endothelial cells and is highly expressed in kidney and has not been detected in testis. On the cytoplasmic surface of occludin, a domain within the C-terminal 150 residues has been shown to bind directly to the well-characterized tight junction protein zona occludens 1 (ZO-1). \n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: Detects a band at ~65kD.\nImmunofluorescence: 2-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nT84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

O5203

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, 50% glycerol. Labeled with FITC.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Anderson, J.M., et al., Curr. Opin. Cell. Biol. 5: 772-778 (1993). 2. Gumbiner, B., Am. J. Physiol. 123: 1631-1633 (1993). 3. Farquhar, M., and Palade, G., J. Cell. Biol. 17: 375-409 (1963). 4. Stevenson, B.R., et al., J. Cell. Biol. 103: 755-766 (1986). 5. Anderson, J.M. et al. (1988) J. Cell. Biol. 106:141-1149. 6. Itoh, M., et al., J. Cell. Biol. 115: 1449-1462 (1993). 7. Gumbiner, B., et al., PNAS USA 88: 3460-3464 (1991). 8. Citi, S., et al., Nature 33: 272-276 (1988). 9. Zhong, Y., et al., J. Cell. Biol. 120: 477-483 (1993). 10. Keno, B.H., et al., J. Cell. Biol. 134: 1003-1018 (1996). 11. Furuse, M., et al., J. Cell Biol. 123: 1777-1788 (1993). 12. Furuse, M., et al., J. Cell. Biol. 127: 1617-1626 (1994). 13. Ando-Akatsuka, Y., et al., J. Cell. Biol. 133: 43-47 (1996).

Additional Info

Recognizes human Occludin Protein. Species Crossreactivity: mouse, rat and canine.