Oct-3, Rat, Control Peptide (Organic Cation Transporter 3)

Control peptide for O5402-07 and O5402-06\n\nMammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus.\n\nOct3 (rat/mouse 551 aa; human 556 aa) share 30% homology with Oct1 and 51% with Oct2. It is most abundant in rat placenta, and moderate in the intestine, heart, and brain. Oct3 expression is very low in kidney and lung and is undetectable in the liver. Oct3 recognized TEA and guanidine along with other cations including neurotoxin (MPP0, neurotransmitter dopamine, and steroids. Oct3 has been identified as extraneuronal monoamine transporter (uptake2).\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\nRecommended Dilution:\nELISA: Coat at 1ug/ml.\nAntibody Blocking: 5-10ug per 1ul O5402-07 (antiserum) or per 1ug O5402-06 (affinity purified antibody).\nOptimal dilutions to be determined by the researcher.\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O5402-05

Size

50ug

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Highly purified

References

1. Kekuda, R., et al., J. Biol. Chem. 273: 15,971-15,979 (1998). 2. Verhaagh, S., et al., Genomics 55: 209-218 (1999). 3. Gruendemann, D., et al., Nat. Neurosci. 1: 349-351 (1998). 4. Wu, X., et al., J. Biol. Chem. 273: 32,776-32,786 (1998). 5. Koespell, H., Ann. Rev. Physiol. 60: 243-266 (1998) (review).

Additional Info

Species Homology:\nMouse and Rat OCT3: 100%\nHuman OCT3: 94%\nRabbit OCT3: 88%