$Oct4, Control Peptide (KLH) (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein)\n$

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Cat no: O5403-01PK

Oct4, Control Peptide (KLH) (POU Domain, Class 5, Transcription Factor 1 Oct3/4, POU5F1, HGNC:9221, MGC22487, Octamer-binding Transcription Factor 3, Oct3, OTF3, OTF4, POU-type Homeodomain-containing DNA-binding Protein)\n

Sequence (linear): RQKGKRSSSDYAQRED [contains an N-terminal Cysteine for KLH conjugation]\n\nOct-4 (POU5F1) is a transcription factor that is expressed in undifferentiated embryonic stem cells and germ cells during normal development (1). A network of key factors that includes Oct-4, Nanog and Sox2, is necessary for the maintenance of pluripotent potential. Downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 has also been shown to be a useful marker for germ cell tumors (4). Stem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development. Oct3/4 maintains developmental potency and governs distinct fates of embryonic stem cells. A moderate increase in expression causes differentiation into primitive endoderm and mesoderm. In contrast, repression of Oct3/4 induces loss of pluripotency and dedifferentiation to trophectoderm. Thus, a critical amount of Oct3/4 is required to sustain stem cell self-renewal, and up- or downregulation induces divergent developmental programs. Data suggest that Oct3/4 is a master regulator of pluripotency that controls lineage commitment.\n\nApplications: \nSuitable for use in ELISA, Antibody Blocking and Antigenic applications. Other applications not tested.\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for 12 months at -20 degrees C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

O5403-01PK

Size

1mg

Applications

ICC, IHC, WB

Reactivities

Hum

Form

Supplied as a liquid in PBS. No preservative added. Conjugated with KLH.

Purity

Highly purified (HPLC, MS)

References

General References:\n1. Zeineddine, D., Papadimou, E., Chebli, K., Gineste, M., Liu, J., Grey, C., Thurig, S., Behfar, A., Wallace, V. A., Skerjanc, I. S. and Puceat, M. Oct-3/4 dose dependently regulates specification of embryonic stem cells toward a cardiac lineage and early heart development. Dev. Cell 11: 535-546, 2006. Niwa, H., Miyazaki, J. and Smith, A. G. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nature Genet. 24: 372-376, 2000. Nichols, J., Zevnik, B., Anastassiadis, K., Niwa, H., Klewe-Nebenius, D., Chambers, I., Scholer, H. and Smith, A. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4. Cell 95: 379-391, 1998.

Additional Info

Recognizes Oct-4. Species Crossreactivity: Human and rodent.