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ORC4L (Origin Recognition Complex Subunit 4, ORC4)

Cat no: O7050-15A


Supplier: United States Biological
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ORC4L is required for initiation of DNA replication with a putative nucleotide triphosphate binding motif. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:8000 Western Blot: 0.5-2ug/ml, human and mouse heart lysates observed on ~50kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: O7050-15A
Reactivities: Human, Mouse, Rat, Bovine, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Johmura Y, Osada S, Nishizuka M, Imagawa M. FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication. J Biol Chem. 2008 Jan 25;283(4):2265-74. 2. Johmura Y, Osada S, Nishizuka M, Imagawa M. FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication. J Biol Chem 283:2265-74 (2008). 3. Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A. Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex. J Biol Chem. 2006 Aug 11;281(32):23264-73. 4. Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A. Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex. J Biol Chem 281:23264-73 (2006). 5. Todorovic V, Giadrossi S, Pelizon C, Mendoza-Maldonado R, Masai H, Giacca M. Human origins of DNA replication selected from a library of nascent DNA. Mol Cell. 2005 Aug 19;19(4):567-75.
Additional info: Recognizes human ORC4L. Species Crossreactivity: mouse. Species sequence homology: Bovine, canine and rat.

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