Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like)

Periostin is a disulfide linked 90kD, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.\n\nHuman OSF-2 His-Tagged Fusion Protein is produced in E. coli, molecular weight is 75kD protein containing 648 amino acid residues of the human OSF-2 and 23 additional amino acid residues-HisTag, Xa-cleavage site.\n\n\nThe amino acid sequence of the recombinant human OSF-2 is 100% homologous to the part of sequence 22-669 aa of the human OSF-2 without signal sequence.\n\nApplications:\nSuitable for use in Western blot and ELISA. Other applications have not been tested. \n\nStorage and Stabillity:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months. Reconstitute with 0.1M acetate buffer pH4. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O8057-05

Size

2ug

Applications

ELISA, WB

Form

Supplied as a lyophilized powder from 0.05M Acetate buffer, pH 4. Reconstitute with 200ul of 0.1M acetate buffer pH4. In higher concentrations the solubility of this antigen is limited.

Purity

~90% (SDS-PAGE). Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.

References

1.Horiuchi K, et al.,. J Bone Miner Res. 1999 Jul; 14(7): 1239-49. 2. Ulstrup JC, et al. Infect Immun. 1995 Feb; 63(2): 672-5. 3.Takeshita S, Kikuno R, Tezuka K, Amann E., Biochem J. 1993 Aug 15; 294 (Pt1): 271-8.