Ovalbumin (Agarose)

Supplier:

United States Biological

Cat no: O8075-02

Ovalbumin (Agarose)

Ovalbumin is a glycoprotein and the major protein of egg white. It has a molecular weight of 43000. Ovalbumin is used for conjugation or immunological determinations as well as drug and pharmaceutical processing.\n\nExpression of genes in E. coli or yeast or baculovirus offers a convenient system to produce large amounts of recombinant proteins that may otherwise be difficult to isolate from natural cells and tissues. Very often antibodies to these newly identified proteins are not available to study its biochemical properties, monitor protein expression, and purification. In order to circumvent this problem, short pieces of well-defined peptides (Poly-His, Flag-epitope or c-myc epitope or HA-tag) or small proteins (bacterial GST, MBP, Thioredoxin, b-Galactosidase, VSV-Glycoprotein etc) are often cloned along with the target gene. Proteins are expressed as fusion proteins. Antibodies to these fusion-tags are already available to monitor fusion protein expression and purification. Therefore, fusion-tags serve as universal tags much like secondary antibodies. Many tags have their own characteristics. Poly-His-fusion proteins (6x His) can bind to Nickel-Sepharose or Nickel-HRP. GST-fusion proteins can bind to glutathione-Sepharose. Therefore, a high degree of purification of fusion protein can be achieved in just one affinity purification step. Purity of fusion proteins can be followed by Tag-antibodies. Very often, fusion proteins are directly injected into animals to generate antibodies. Some fusion tags can be removed later by treatment with enzymes to generate tag-free recombinant proteins.\n\nApplications: \nSuitable for use in antibody purification. Other applications not tested.\n\nRecommended Dilution:\nRegeneration:\nWash with 3ml of 0.1M glycine buffer, pH 2.5, and then immediately washing with PBS pH 7.4 with 10-20 volumes. Store column in PBS containing 0.05% azide at 4 degrees C. DO NOT FREEZE the beads at any stage.Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nStored at 4 degrees C. Do not freeze. Aliquots are stable for at least 6 months from date of opening.

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SPECIFICATIONS

Catalog Number

O8075-02

Size

1ml

Conjugates

Agarose

Form

Supplied as a suspension in PBS, pH

Alternative Names

Egg white

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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