Oxytocin, Rat Control Peptide

Control Peptide for O8250-15 (affinity purified) and O8250-20 (antiserum).\n\nRat OT is 9 hypothalmic peptide (1).\n\nThe hypothalamic Oxytocin (OT) is a nine-amino acid peptide, which exerts multiple biological actions as a hormone and as neurotransmitter. OT stimulates uterine smooth muscle and mammary myoepithelial cell contraction, prostaglandin production by uterine endometrial and amnion cells, milk ejaculation from the mammary gland, and induction of specific mating behavior and maternal behaviors. Just before the onset of labor, uterine myometrium becomes extremely sensitive to oxytocin, for which it is a primary target tissue, because of a dramatic increase in the number of oxytocin receptors. OT initiates its physiological activity by interacting with the G protein-coupled receptor (GPCR) known as oxytocin receptor (OTR). The encoded receptor is a 388-amino-acid polypeptide with 7 transmembrane domains typical of G protein-coupled receptors. Messenger RNAs for the receptor are of two sizes, 3.6 kilobases in breast, and 4.4 kilobases in ovary, uterine endometrium and myometrium. The mRNA level in the myometrium is very high at term.\n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot due to low MW. Other applications not tested. \n\nRecommended Dilution: \nELISA: 1ug/ml to coat plates.\nAntibody Blocking: 5-10ug peptide.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

O8250-25

Size

50ug

Reactivities

Hum, Rat

Form

Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.

Purity

Highly purified

References

References:\n\n1. Rehbein M et al (1986) Biol Chem. Hoppe-Seyler 367, 695-704; Ivell R et al (1984) PNAS 81, 2006-2010; Mohr E et al (1988) FEBS Lett. 242, 144-148; Vhauvez MT et al (1981) BBRC 103, 595-603; Schlesinger DH et al (1980) FEBS Lett. 80, 371-373; Hara Y et al (1990) Brain res. Mol Brain res. 8, 319-324; ozen F et al (1995) PNAS 92, 200-204; Kumura T et al (1992) Nature 356, 526-529

Additional Info

Species sequence homology: mouse, rat, human, bovine, sheep 100%.