p-NPP is a single component reagent system for microwell assay of Calf Intestine Alkaline Phosphatase (CIAP or CAP) labeled probes and is an excellent substrate for phosphatase based ELISA assays.
p-NPP produces a soluble end product which is yellow in color and read at 405-410 nm. Both kinetic and end point determinations can be performed.
p-NPP is the customary phosphate ester used as a substrate for measuring AP activity due to its high solubility in alkaline buffers which affords zero order kinetics and significant molar extinction coefficient of 18600 mol-1cm-1.
Storage and Stability:
Stable for 12 months at 4 degrees C.
Also Available:
N2600: p-Nitrophenyl Phosphate (pNPP)
N2600-10: p-Nitrophenyl Phosphate (pNPP), 50X Concentrate, 0.19M/Liter
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