P53 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human P53 in cell lysates.\n\nP53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human P53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kilodalton (kD) protein on SDS-PAGE. The pattern of p53 splicing was specific for brain areas and for individuals. And human kidney and heart expressed only full-length p53. It has played a vital role in conserving stability by preventing genome mutation. \n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nK7PPA8\n\nEntrez Gene ID:\n7157\n\nKit Components:\n96-well plate pre-coated with anti-P53, Human\nLyophilized recombinant P53, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144003

Size

96Tests

References

1. Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L, Benchimol S (December 1984). \"Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene\". EMBO J. 3 (13): 3257