p53, phosphorylated BioAssay(TM) Sample Kit

Supplier:

United States Biological

Cat no: P1001-25

p53, phosphorylated BioAssay(TM) Sample Kit

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to reduced interaction of p53 with its negative regulator, oncoprotein MDM2 (4). MDM2 inhibits the accumulation of p53 by targeting it for ubiquitination and proteasomal degradation (6,7).\n\np53 can apparently be phosphorylated by ATM, ATR and DNA-PK at Ser15; this phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage (4,5). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability and activity (8,9). p53 is phosphorylated at Ser392 in vivo (11,12) and by CAK in vitro (12). Phosphorylation of p53 at Ser392 is altered in human tumors (14) and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53 (10,11,13). p53 is phosphorylated at Ser6 and Ser9 by CK1d and e both in vitro and in vivo (10,15). Serine 46 is important in regulating the ability of p53 to induce apoptosis (16).\n\nThe Phospho-p53 Antibody Sampler Kit provides a fast and economical means to evaluate p53 phosphorylation at multiple sites, including Ser6, Ser9, Ser15, Ser20, Ser37, Ser46 and Ser392.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nSee protocol\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1001-25

Size

9x40ul

Applications

ELISA, ICC, WB

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol.

Purity

Purified by protein A and peptide affinity chromatography.

References

(1) Levine, A.J. (1997) Cell 88, 323

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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