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p53, phosphorylated (Ser392) (Tumor Suppressor Protein, Oncogene Protein)

Cat no: P1001-26A4

p53, phosphorylated (Ser392) (Tumor Suppressor Protein, Oncogene Protein)

Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP \n\nFusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of a mouse SP-2 myeloma cell line\n\nImmunohistochemistry: * This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended positive control tissue: Colon or breast carcinoma.\n\nApplications:\nImmunohistochemistry (paraffin)\nImmunoprecipitation\nWestern Blot

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SPECIFICATIONS

Catalog Number

P1001-26A4

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Blaydes, J.P. and Hupp, T.R. (1998). DNA damage triggers DRB-resistant phosphorylation of human p53 at the CK2 site. Oncogene 17: 1045-1052.

Additional Info

Recognizes human p53, specifically binding only to p53 phosphorylated at the Ser392 residue. Ser392 phosphorylation of p53 has been shown to occur following DNA damage. Phosphorylation of p53 at this site may be mediated the casein kinase 2 (CK2) and also be other phosphorylation mechanisms. In Immunohistochemistry this antibody generally stains 5-10% of cells labelled with antibodies specific for non-phosphorylated p53.

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