p53, Recombinant, Human (Tumor Suppressor Protein, Oncogene Protein)

p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6; protein p53 senses DNA damage and possibly facilitates repair. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.\n\nRecombinant Human p53 is a non-glycosylated polypeptide chain that migrates as a 53kD protein on SDS-PAGE. However, based on calculations from its amino acid residues, p53's mass is in fact only 43.7kDa. This difference is attributed to the high number of proline residues in the protein which slow its migration on SDS-PAGE, consequently making it appear larger than it actually is.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Human p53.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P1001-26S

Size

2ug

Form

Supplied as a liquid in 20mM Tris, pH 8, 0.6M sodium chloride, and 50% glycerol.

Purity

(same/more than) 95% by SDS-PAGE. Chromatographically purified.

References

1. Fbxw7/Cdc4 is a p53-dependent, haploinsufficient tumour suppressor gene. Mao JH, Perez-Losada J, Delrosario R, Bryson S, Nature 2004 Dec 9;432(7018):775-9 2. The BCL6 proto-oncogene suppresses p53 expression in germinal-centre B cells. Phan RT, Dalla-Favera R, Nature 2004 Dec 2;432(7017):635-9 3. DNA damage-induced downregulation of Cdc25C is mediated by p53 via two independent mechanisms: one involves direct binding to the cdc25C promoter. St Clair S, Giono L, Resnick-Silverman L, DaCosta A, Mol Cell 2004 Dec 3;16(5):725-36 4. C/EBPalpha is a DNA damage-inducible p53-regulated mediator of the G1 checkpoint in keratinocytes. Yoon K, Smart RC, Mol Cell Biol 2004 Dec;24(24):10650-60 5. Selective transcription of p53 target genes by zinc finger-p53 DNA binding domain chimeras. Falke D, Fisher MH, Biochim Biophys Acta 2004 Nov 24;1681(1):15-27 6. p53 and DPC4 alterations in the bile of patients with pancreatic carcinoma. Pantalone D, Pelo E, Giotti I, Nesi G, J Surg Oncol 2004 Dec 15;88(4):210-6.