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Pacific Blue(TM) anti-mouse CD45

Pacific Blue(TM) anti-mouse CD45

Cat no: 103125


Supplier: BioLegend
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CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Catalogue number: 103125
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 25 microg
Clone: 30-F11
Gene: 19264
Dilutions: This CD45 reagent is developed for immunofluorescent staining for flow cytometric analysis, the suggested use of this reagent is ? 0.25 microg per 106 cells in 100 microl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.* Pacific Blue(TM) has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue(TM) conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. ** Pacific Blue(TM) is a registered trademark of Molecular Probes, Inc. Pacific Blue(TM) dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents.
Storage buffer: The CD45 antibody solution should be stored undiluted at 4C, and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.5 mg/ml
Antigen: Mouse thymus or spleen
Isotype: Rat IgG2b, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: T200, Ly-5, LCA
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