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PaeI (SphI)

Cat no: P1800

PaeI (SphI)

Sequence:\n5'-G C A T G^C-3' \n3'-C^G T A C G-5'\n\nSource: Pseudomonas aeruginosa\n\nConcentration: 10u/ul \n\nUnit Definition: \nOne unit is defined as the amount of PaeI required to digest 1ug of lambda DNA fragments in 1 hr at 37 degrees C in 50ul assay buffer.\n\nDiluent Buffer: \nSupplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA, 50% glycerol.\n\nStorage Buffer: \n10mM potassium phosphate (pH 7.0 at 25 degrees C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/ml BSA, 0.15% Triton X-100 and 50% glycerol.\n\nSupplied with:\nR1625: Supplied as a liquid in 33mM Tris-acetate, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA, pH 7.9 at 37C. \n\nR1625-01: Restriction Enzyme Buffer B, 10X: Supplied as a liquid 10mM Tris-HCl, pH 7.5 at 37 degrees C, 10mM magnesium chloride, 0.1mg/ml BSA. Incubate at 37 degrees C\n\nDouble Digests:\nR1625 is provided to simplify buffer selection for double digests. 98% of PaeI, works well in a 1x or 2x.

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SPECIFICATIONS

Catalog Number

P1800

Size

500U

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