PAK1 (E195) pAb

Three isoforms of serine/threonine kinases, designated (alpha)PAK p68, (beta)PAK p65 and (gamma)PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The (alpha), (beta) and (gamma)PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on (gamma)PAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with (gamma)PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

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SPECIFICATIONS

Catalog Number

BS1803

Size

100ug/100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 180-230 of Human PAK1.

Species

PAK1 (E195) pAb detects endogenous levels of PAK1 protein.

Dilutions

WB: 1:500~1:1000\nIHC: 1:50~1:200\nIF: 1:50~1:200

Swiss Prot

Q13153

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul