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Pap Pen, Mini

Cat no: P3102-50

Pap Pen, Mini

The Mini PAP pen draws a water repellent circle around slide mounted tissue, preventing the waste of valuable reagents by keeping the liquid pooled in a single droplet. The Mini PAP Pen is especially useful when working with multiple sections on a single slide because its tip is smaller than the PAP pen. Each pen can be used to draw about 400 circles.\n\nApplications: \nSuitable for use in Immunohistochemistry and fluorescent staining methods. Other applications not tested.\n\nRecommended Usage:\n1) For Paraffin-embedded sections: \n- Deparaffinize and rehydrate tissue sections.\n- Rinse tissue sections with PBS and wipe off excess liquid around the tissue.\n- Draw a circle on the glass slide around the section with Mini PAP pen.\n- Dry the circle for 1-2 minutes at RT.\n- Continue the normal immunohistostaining process.\n\n2) For Frozen sections: \n- Preclean glass slide.\n- Coat slide with H5107: HistoGrip (50X concentrate) or other adhesive media (Optional).\n- Cut and mount tissue sections on the slides.\n- Rinse tissue sections with PBS and wipe off excess liquid around the tissue.\n- Draw a circle on the glass slide around the section with Mini PAP pen.\n- Dry the circle for 1-2 minutes at RT.\n- Continue the normal immunohistostaining process.\n\nNote: The water repellent circle is removable in xylene or xylene substitutes. Since it is not influenced by water soluble media, it will not interfere with microscopic study.\n\nStorage and Stability:\nStore at RT with cap on tight and tip pointed down. Press down on tip when pen starts to dry out.

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SPECIFICATIONS

Catalog Number

P3102-50

Size

1 Ea

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