Papain, Lyophilized

Supplier:

United States Biological

Cat no: P3100-01

Papain, Lyophilized

Proteolytic enzymes are widely used in cell isolation. With some tissues papain has proved less damaging and more effective than other proteases. Papain is a sulfhydryl protease from Carica papaya latex. Native crystalline papain is quite unreactive until acted upon by mild reducing agents such as cysteine or cyanide. It may exist as a zymogen (Brocklehurst and Kierstan, 1973). For a general review, see Liener (1974). Papain has wide specificity. It will degrade most protein substrates more extensively than the pancreatic proteases. It is also an esterase. The action of papain on leucine methyl ester produces an insoluble polyleucine peptide. Papain breaks down the intercellular matrix of cartilage.\n\nPapain is a single peptide chain of 211 residues folded into two parts that form a cleft. A three-dimensional structure has been indicated. The molecule has one free SH group which is functional. There are seven subsites, each is capable of accommodating a single amino acid residue of a peptide substrate.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Reconstitute with sterile buffer. Store at 4 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3100-01

Size

25mg

Form

Supplied as a lyophilized powder prepared from a 2X crystalline suspension

References

US Biological application reference: Yang, X. et al., (2013) BioResearch Open Access, doi:10.1089/biores.2013.0014. 1. Akahane, K., and Umeyama, H.: Binding Specificity of Papain and Cathepsin B, Enzyme 36: 141, 1986. 2.

Alternative Names

EC=3.4.22.2; Papaya latex

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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