Parathyroid Hormone, intact (PTHi) BioAssay(TM) ELISA Kit

PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre- proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes1. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and mid-region fragments. In subjects with renal insufficiency, C-terminal and mid- region PTH assays typically give elevated PTH results, as reflected by impaired renal clearance2.\n\nIntended Use:\nThe Intact-PTH ELISA is intended for the quantitative determination of Intact-PTH (Parathyroid Hormone) in human serum. This assay is intended for in vitro diagnostic use.\n\nPrinciple of the Test:\nThe Intact PTH Immunoassay is a two-site ELISA [Enzyme-Linked ImmunoSorbent Assay] for the measurement of the biologically intact 84 amino acid chain of PTH. Two different goat polyclonal antibodies to human PTH have been purified by affinity chromatography to be specific for well defined regions on the PTH molecule. One antibody is prepared to bind only the mid-region and C-terminal PTH and this antibody is biotinylated.\n\nStreptavidin Well

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SPECIFICATIONS

Catalog Number

167563

Size

96Tests

Applications

ELISA

References

1. Segre,G.V.,NiallH.D.,HabenerJ.F.et.al.:Metabolismofparathyroidhormone:physiologicalandclinical significance. Am. J. Med. 56: 774,1974 2. Mallete, L.E., Gagel, R.F.: Parathyroid Hormone and Calcitonin. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 65-69, 1990. 3. Bilezikian, J.P.: Primary Hyperparathyroidism. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 109-111, 1990. 4. Stewart, A.F.: Humoral Hypercalcemia of Malignancy. In: Murray J.F. (ed) Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism. American Society for Bone and Mineral Research, Kelseyville; William Byrd Press, Richmond, pp. 115-118, 1990. 5. Mallette, L.E.: The parathyroid polyhormones: New concepts in the spectrum of peptide hormone action. Endocrin. Rev. 12:110-117, 1991. 6. Kruger, L.., Rosenblum, S., Zaazra, J. and Wong, J. Intact PTH is stable in unfrozen EDTA plasma for 48 hours prior to laboratory Analysis. Clin. Chem. 41:6: page S47, 1995.