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Paxillin (PXN)

Cat no: P3113-90D

Paxillin (PXN)

Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p120BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion, consistent with the belief that paxillin is involved in the regulation of cell morphology. Additionally because of is SH3-binding domain, paxillin associates tightly with FAK and Crk in a manner that is independent of the extracellular matrix. Although paxillin was initially detected in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 1:50-1:100\nImmunoprecipitation: 2ug/mg\nImmunohistochemistry: Frozen\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nColon carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3113-90D

Size

500ul

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Rat, Bov

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. De Curtis & Malanchini Expt Cell Res 230: 233, 1997. 2. Hanks & Polte Bioessays 19: 137, 1997. 3. Leventhal et al. J Biol Chem 272: 5214, 1997.

Additional Info

Recognizes human Paxillin. Species Crossreactivity: bovine, avian, rat.

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