PCSK9 is a serine protease of the protein convertase family having profound effects on plasma low-density lipoprotein (LDL)-cholesterol (LDL-C) levels through its ability to mediate LDL receptor (LDLR) protein degradation. The protein slows down the uptake of cholesterol by degrading LDLR after they have internalized LDL and thus plays a significant role in cholesterol homeostasis. PCSK9 also potentially averts excessive cholesterol accumulation within the cell by preventing the recycling of LDLRs to the cell surface. Structurally, the protein includes a signal peptide, followed by a prodomain, a subtilisin-like catalytic domain, and a variable C-terminal domain. The prodomain serves a dual role as a chaperone for folding and as an inhibitor of catalytic activity. Human PCSK9 gene is localized in the chromosomal region 1p32.3. Several mutations (p.S127R, p.F216L, p.D374Y) of this gene have been reported to cause hypercholesterolemia.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:16,000
Immunohistochemistry (Paraffin): 2-6ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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