pDC TLR9 (Plasmacytoid Dendritic Cells, Toll Like Receptor 9) BioAssay(TM) Kit

Supplier:

United States Biological

Cat no: P3120-59D

pDC TLR9 (Plasmacytoid Dendritic Cells, Toll Like Receptor 9) BioAssay(TM) Kit

Plasmacytoid Dendritic Cell (pDC)/TLR9 Kit is validated by Flow Cytometry for identifying human TLR9 expression in pDC from human whole blood, freshly isolated peripheral blood mononuclear cells (PBMC) and frozen PBMC.\n\nPlasmacytoid Dendritic Cells (pDC) are a subset of peripheral blood Dendritic Cells which play an important role in both innate and adaptive immune responses. pDC recognize viral components via Toll-like Receptors (TLR) including TLR7 and TLR9, and then rapidly produce Interferon-alpha (IFNa) as a primary anti-viral immune response. pDC also participate in antigen presentation, an important functional role which drives the adaptive immune response. Intense investigation has led to pDC identification with a unique set of markers by flow cytometry. pDC are usually present in relatively low frequency in peripheral blood. Direct identification of subsets of pe- ripheral blood DCs has an advantage in analyzing changes in number or function of DC subsets during chronic viral infections or diseased patients, as examples.\n\nKit Contents:\nhLMAX: Human Lineage Marker Antibody Mix\n(human CD3, CD14, CD16, CD19, CD20, CD56, HLA-DR antibodies)*\n10ul/test, 300ul\n* CD3, CD14, CD16, CD19, CD20 and CD56 are conjugated to FITC, HLA-DR is conjugated to PerCP-Cy5.5.\n\nCD123 AF647 (human CD123 Alexa Fluor 647 conjugate) antibody\n5ul/test, 125ul\n\nTLR9 PE (human TLR9 PE conjugate) antibody\n5ul/test, 125ul\n\nMouse IgG1 Isotype control Alexa Fluor 647 conjugate antibody\n5ul/test, 125ul\n\nMouse IgG1 Isotype control PE conjugate antibody\n5ul/test, 125ul\n\nStaining buffer (1X)\n2 x 60ml\n\nFixation buffer (1X)\n60ml\n\nPermeabilization buffer (10X)\n2 x 60ml\n\nStorage Conditions: Store at 4 degrees C, do not freeze; conjugated antibody is light-sensitive\n\n

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SPECIFICATIONS

Catalog Number

P3120-59D

Size

1Kit

Applications

FC

References

1. Kadowaki, N., S. Antonenko, J.Y. Lau, and Y.J. Liu. 2000. J Exp Med 192:219

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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