PDE10A, BioAssay(TM) Kit (cAMP and cAMP-inhibited cGMP 3',5'-cyclic Phosphodiesterase 10A)

Supplier:

United States Biological

Cat no: 137324

PDE10A, BioAssay(TM) Kit (cAMP and cAMP-inhibited cGMP 3',5'-cyclic Phosphodiesterase 10A)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE10A is a dual substrate PDE highly expressed in striatal medium spiny neurons. PDE10A inhibitors can improve the cognitive symptoms of schizophrenia, and exhibit potential therapeutic value for Huntington's Disease. The PDE10A Assay Kit is designed for identification of PDE10A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A to the binding agent.\n\nIntended Use:\nDesigned for identification of PDE10A inhibitors using fluorescence polarization.\n\nTest Principle:\nThe PDE10A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE10A enzyme, fluorescently labeled PDE10A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE10A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.\n\nKit Components:\nPDE10A2: 1x700ng\nFAM-Cyclic-3

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SPECIFICATIONS

Catalog Number

137324

Size

100Tests

References

1. Threlfell S, et al. (2009) Pharmacol Exp Ther 328(3):785-95. 2. Menniti FS, et al. (2007) Curr Opin Investig Drugs 8(1):54-9.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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