PDE11A Assay Kit, BioAssay(TM)

Supplier:

United States Biological

Cat no: 137326

PDE11A Assay Kit, BioAssay(TM)

Phosphodiesterases (PDEs) are involved in the dynamic regulation of cAMP and cGMP signaling. PDE11A has been observed in skeletal muscle, prostate, testis, salivary gland, thyroid gland, and liver. PDE11A may play role in the CNS with an association to major depressive disorder.\n\nIntended Use:\nThe PDE11A Assay Kit is designed for identification of PDE11A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE11A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.\n\nTest Principle:\nThe PDE11A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE11A enzyme, fluorescently labeled PDE11A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE11A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE11A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE11A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.\n\nKit Components:\nNUNC microtiter plate, 1x96wells\nRecombinant enzyme, 1x1ug\nFAM-Cyclic-3

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SPECIFICATIONS

Catalog Number

137326

Size

96Tests

References

1. Wong, M.L., et al., Proc Natl Acad Sci USA 103(41): 15,124-15,129 (2006).

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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