PDE1B, Active, Recombinant, Rat, His-GST Tag (Calcium/Calmodulin-dependent 3',5'-cyclic Nucleotide Phosphodiesterase 1B, Cam-PDE 1B, 63kD Cam-PDE, Pde1b1)

PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate.\n\nSource:\nRecombinant corresponding to full length rat PDE1B, fused to His-tag at N-terminal, expressed in Sf9 insect cells via a baculovirus expression system.\n\nMolecular Weight:\n~89kD\n\nSpecific Activity:\n~3600pmol/min/ug\n\nUnit Definition:\nOne unit is defined as the amount of enzyme that will convert 1pmole of 3?, 5?-cAMP to 5?-AMP per min at 37 degrees C.\n\nApplications: \nSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137328

Size

10ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mMImidazole, 3mM DTT, 20% glycerol.

Purity

Purified (~70%)

References

1. Bender A.T. and Beavo J.A. Proc. Natl. Acad. Sci. U.S.A. 103 (2), 460-465 (2006). 2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).